\u4ee5\u4e0b\u6240\u5217\u662f\u672c\u7814\u7a76\u5ba4\u8fd1\u5e74\u4e4b\u7814\u7a76\u4e3b\u984c\u8207\u4ee3\u8868\u6027\u6210\u679c\uff0c\u5305\u62ec\u5b78\u751f\u53c3\u8207\u4e4b\u7814\u7a76\u8a08\u756b\u3001\u8de8\u9818\u57df\u5408\u4f5c\uff0c\u4ee5\u53ca\u5fae\u751f\u7269\u57fa\u56e0\u9ad4\u5b78\u8207\u9577\u8b80\u9577\u5b9a\u5e8f\u6280\u8853\u65bc\u81e8\u5e8a\u8207\u751f\u7269\u5b78\u554f\u984c\u4e0a\u7684\u61c9\u7528\u3002The themes summarized below reflect the evolving research directions of the laboratory in recent years, including representative projects, collaborative studies, and student-involved publications. These research activities involve graduate students, interdisciplinary collaborations, and the application of comparative genomics and long-read sequencing technologies to clinically and biologically important microbial systems.<\/p>\n\n\n\n
Research in this area has focused on the role of mobile genetic elements \u2014 including plasmids, integrative and conjugative elements, resistance islands, and transposons \u2014 in the emergence, dissemination, and evolution of antimicrobial resistance among clinically important bacteria.<\/p>\n\n\n\n
Studies from the laboratory have included comparative genomics of multidrug-resistant pathogens, characterization of resistance-associated mobile elements, and investigation of regulatory mechanisms linking antimicrobial resistance with bacterial adaptation and virulence.<\/p>\n\n\n\n
One major research direction in the laboratory has focused on the role of plasmid-associated regulatory elements in antimicrobial resistance and bacterial adaptation.
An early study identified RamAp, an IncHI2 plasmid-borne efflux pump regulator in Salmonella enterica, demonstrating how horizontally acquired regulatory genes may contribute to antimicrobial resistance and bacterial physiology.
This project was initially conducted by Hong YP, an in-service doctoral trainee from the Taiwan Centers for Disease Control (CDC), and was later expanded through functional and regulatory studies involving graduate students from the laboratory, including Yeh WS, Wan MS, Wu YC, and laboratory alumnus Hong YM.
Subsequent work further investigated downstream gene regulation and the functional roles of RamAp in bacterial physiology, oxidative stress response, motility, and virulence, extending the project from plasmid-associated antimicrobial resistance toward broader questions of bacterial adaptation and pathogenicity.<\/p>\n\n\n\n
Research in this area focuses on the genomic epidemiology of clinically important bacterial pathogens using comparative genomics and whole-genome sequencing approaches.<\/p>\n\n\n\n
Studies from the laboratory have investigated hospital-associated outbreaks, dissemination of high-risk bacterial lineages, and the genomic characteristics of emerging pathogens, including Elizabethkingia anophelis<\/em>, methicillin-resistant Staphylococcus aureus<\/em> (MRSA), and Mycoplasma pneumoniae<\/em>.<\/p>\n\n\n\n The laboratory works in close collaboration with clinician-scientists and in-service doctoral trainees from affiliated hospitals, integrating clinical microbiology, infectious disease practice, and comparative genomics approaches for the investigation of hospital-associated pathogens and emerging bacterial lineages.<\/p>\n\n\n\n One major research direction in this area has focused on the genomic epidemiology and hospital-associated transmission of Elizabethkingia anophelis<\/em>, an emerging opportunistic pathogen associated with healthcare settings.<\/p>\n\n\n\n This project originated through collaboration with clinician-scientists and medical laboratory professionals from Changhua Christian Hospital, particularly Lee YL (infectious disease physician and in-service doctoral trainee) and Chang HL (medical technologist and graduate student). Subsequent studies involved graduate students from the laboratory, including Liu KM, Liao YC, Huang JC, Yao YC, Li RY, Hsu MH, and Lin YZ.<\/p>\n\n\n\n Research from this project identified a prolonged hospital-associated outbreak linked to contaminated water systems and further characterized the genomic evolution and lineage diversification of Elizabethkingia anophelis<\/em> strains in Taiwan. Additional studies explored genomic islands and efflux pump-associated tolerance to disinfectants in outbreak-associated strains.<\/p>\n\n\n\n The project also led to the characterization of the unusual Elizabethkingia<\/em> genomospecies 2-6 lineage and multiple conference presentations at the Molecular Biology Society of Japan (MBSJ), including poster and oral presentations by graduate students from the laboratory.<\/p>\n\n\n\n For more: \u6709\u4e9b\u7d30\u83cc\u4e0d\u662f\u7a81\u7136\u51fa\u73fe\uff0c\u800c\u662f\u6211\u5011\u73fe\u5728\u624d\u770b\u5f97\u61c2<\/a><\/p>\n\n\n\n Another major research direction has focused on the comparative genomics and evolution of methicillin-resistant Staphylococcus aureus<\/em> (MRSA), particularly human-adapted CC398 and ST1232 lineages circulating in Taiwan.<\/p>\n\n\n\n This project was primarily led by Wu TH, a pediatrician from Show Chwan Memorial Hospital and in-service doctoral trainee in the laboratory, together with graduate student Wu YC. Additional contributions were made by laboratory members including Li RY, Wan MS, and Yeh WS.<\/p>\n\n\n\n Studies from this project investigated the genomic characteristics, phylogenetic relationships, and mobile genetic elements associated with emerging MRSA lineages, with particular emphasis on SCCmec structures, prophage-associated virulence factors, and the adaptation of livestock-associated MRSA lineages to human hosts.<\/p>\n\n\n\n In addition to peer-reviewed publications, this research direction has also been presented at the Molecular Biology Society of Japan (MBSJ) through poster and oral presentations by both doctoral and graduate students from the laboratory.<\/p>\n\n\n\nGenomic epidemiology of Elizabethkingia anophelis<\/em><\/h3>\n\n\n\n
Related publications<\/h3>\n\n\n\n
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Comparative genomics of human-adapted MRSA CC398 lineages<\/h3>\n\n\n\n