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\u9664\u4e86\u4f7f\u7528\u4e0a\u7c21\u55ae\u4fbf\u5229\uff0c\u7701\u6389\u4e86\u8a31\u591a\u5de5\u4f5c\u4e0a\u7684\u9ebb\u7169\u4e4b\u5916\uff0c\u5b83\u7684\u8f38\u5165\u5177\u6709\u76f8\u7576\u7684\u5f48\u6027\u3002\u5982\u679c\u4f60\u7684\u6563\u5f48\u69cd\u5b9a\u5e8f\u53ea\u6709Illumina\u7684\u77ed\u8b80\u53d6\u8cc7\u6599\u7684\u8a71\uff0c\u5b83\u53ef\u4ee5\u900f\u904e\u512a\u5316\u7684SPAdes\u6d41\u7a0b\u4f86\u7522\u751f\u7d44\u88dd\u7d50\u679c\u3002\u6216\u8005\uff0c\u5982\u679c\u4f60\u53ea\u6709\u9577\u8b80\u53d6\u8cc7\u6599(PacBio\u6216Nanopore)\u7684\u8a71\uff0cUnicycler\u6703\u9760miniasm\u548cRacon\u4f86\u9032\u884c\u5e8f\u5217\u7684\u7d44\u88dd\u3002\u800c\u82e5\u662f\u77ed\u8b80\u53d6\u5e8f\u5217\u548c\u9577\u8b80\u53d6\u5e8f\u5217\u7684\u8cc7\u6599\u90fd\u6709\u7684\u8a71\uff0c\u9019\u500b\u8edf\u9ad4pipeline\u7684\u6df7\u5408\u7d44\u88dd(\u4e5f\u90fd\u662f\u81ea\u52d5\u7684)\u901a\u5e38\u6703\u8b93\u4f60\u5f97\u5230\u8b93\u4eba\u9a5a\u8277\u7684\u7d44\u88dd\u7d50\u679c\u3002\u4f7f\u7528unicycler\u4f5c\u6563\u5f48\u69cd\u5e8f\u5217\u6df7\u5408\u7d44\u88dd\u7684\u6642\u5019\uff0c\u5c0d\u9577\u5e8f\u5217\u7684\u8cea\u8207\u91cf\u8981\u6c42\u4f3c\u4e4e\u90fd\u4e0d\u9ad8\u3002\u6240\u4ee5\u53ea\u8981\u4f60\u6709\u505a(\u4e0d\u9700\u8981\u592a\u597d)\u7684\u9577\u8b80\u53d6\u5e8f\u5217\u5b9a\u5e8f\uff0c\u901a\u5e38\u52a0\u4e0a\u9019\u4e9b\u9577\u5e8f\u5217\uff0cunicycler\u5c31\u53ef\u4ee5\u8b93\u4f60\u539f\u672c\u53ea\u9760\u77ed\u5e8f\u5217\u7522\u51fa\u7684\u7d44\u88dd\u7d50\u679c\u5728\u5b8c\u6210\u5ea6\u4e0a\u7372\u5f97\u975e\u5e38\u5927\u7684\u63d0\u5347\u5594\u3002<\/p>\n\n\n\n \u6211\u5011\u539f\u672c\u90fd\u662f\u5728\u8dd1Linux\u7684\u5de5\u4f5c\u7ad9\u5e95\u4e0b\u505a\u7d44\u88dd\uff0c\u4f46\u5176\u5be6\u96fb\u8166\u786c\u9ad4\u7684\u898f\u683c\u9084\u662f\u8981\u770b\u4f60\u7684\u5206\u6790\u8edf\u9ad4\u3001\u9084\u6709\u8cc7\u6599\u91cf\u4f86\u6c7a\u5b9a\uff0c\u4ee5\u7d30\u83cc\u4f86\u8aaa\u57fa\u56e0\u9ad4\u96d6\u5c0f\uff0c\u5982\u679c\u4f60\u50bb\u50bb\u7684\u62ff\u4e94\u767e\u500d\u6216\u4e00\u5343\u500d\u7684\u6563\u5f48\u69cd\u77ed\u5e8f\u5217\u53bb\u8dd1\u7d44\u88dd\uff0c\u90a3\u9ebc\u7576\u7136\u6703\u89ba\u5f97\u96fb\u8166\u4e0d\u5920\u8dd1(\u5176\u5be6\u662f\u8166\u888b\u4e0d\u5920…)\u3002\u73fe\u5728\u6211\u5011\u5728\u7814\u7a76\u5ba4\u90fd\u7528iMac\u4f86\u8dd1\u6563\u5f48\u69cd\u5e8f\u5217\u7d44\u88dd(\u4e2d\u4f4e\u968e\u7684\u898f\u683c)\uff0c\u860b\u679c\u6559\u80b2\u5546\u5e97\u7684\u539f\u5ee0\u898f\u683c\u5c31\u9060\u9060\u5920\u7528\u4e86\u3002<\/p>\n\n\n\n \u6211\u5011\u662f\u7528Anaconda\u4f86\u7ba1\u7406python\u7684\u74b0\u5883\uff0cAnaconda\u662f\u958b\u6e90\u7684\uff0c\u5b83\u5305\u542b\u4e86\u8a31\u591a\u5e38\u7528\u7684Python\u5957\u4ef6\u548c\u5de5\u5177\uff0c\u5b83\u6703\u81ea\u52d5\u8655\u7406\u5957\u4ef6\u4e4b\u9593\u7684\u4f9d\u8cf4\u95dc\u4fc2\uff0c\u4e26\u78ba\u4fdd\u76f8\u5bb9\u6027\u3002Anaconda\u9084\u63d0\u4f9b\u4e86Python\u7684\u74b0\u5883\u7ba1\u7406\u5de5\u5177\uff0c\u53ef\u4ee5\u65b9\u4fbf\u5730\u7ba1\u7406\u4e0d\u540c\u7248\u672c\u7684Python\u548c\u8edf\u9ad4\u5957\u4ef6\u3002Anaconda\u53ef\u4ee5\u5728\u9019\u88e1\u4e0b\u8f09\uff1ahttps:\/\/www.anaconda.com\/distribution\/<\/a><\/p>\n\n\n\n \u6709\u4e86Anaconda\uff0c\u5728Linux\u6216Mac\u88e1\u9762\u5b89\u88ddunicycler\u5c31\u5f88\u7c21\u55ae\u4e86\uff1ahttps:\/\/anaconda.org\/bioconda\/unicycler<\/a><\/p>\n\n\n\n \u77ed\u8b80\u53d6\u5e8f\u5217\u8207\u9577\u8b80\u53d6\u5e8f\u5217\u7684\u6df7\u5408\u7d44\u88dd\u6642\uff0c\u4f86\u81eaIllumina\u7684fastq\u6a94\uff0c\u8981\u7528\u58d3\u7e2e\u904e\u7684.fastq.gz\u6a94\u6848\uff0c\u6216\u8005\u662f.fastq\u90fd\u53ef\u4ee5\u3002\u56e0\u70ba\u662fpair-end reads\uff0c\u4f60\u6703\u6709\u5169\u500b\u9019\u6a23\u7684\u77ed\u5e8f\u5217\u5b9a\u5e8f\u7d50\u679c\u7684\u6a94\u6848\uff0c\u901a\u5e38\u6a94\u540d\u6703\u5beb\u50cf\u662fread_1\u8ddfread_2\u9019\u6a23\u3002\u9577\u5e8f\u5217\u7684\u8a71\uff0c\u4ee5nanopore\u4f86\u8aaa\uff0c\u5c31\u628abasecalling\u4e4b\u5f8c\u7684fastq\u6a94\u6848\u62ff\u4f86\uff0c\u7528cat\u6307\u4ee4\u52a0\u5728\u4e00\u8d77(\u9069\u91cf\u5730)\u9935\u7d66\u4ed6\u5c31\u597d\u3002\u4e0b\u4e86\u50cf\u9019\u6a23\u7684\u6307\u4ee4\uff0c\u7a0d\u7b49\u5c31\u597d\u4e86\uff1a<\/p>\n\n\n\n \u5f9e\u6563\u5f48\u69cd\u7684FASTQ\u7522\u51fa\u300c\u4e00\u500b\u5708\u5708\u300d\u7684\u597d\u5de5\u5177<\/p>\n","protected":false},"author":1,"featured_media":1696,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"colormag_page_container_layout":"no_sidebar_full_width","colormag_page_sidebar_layout":"no_sidebar","footnotes":""},"categories":[5],"tags":[15,14,30],"class_list":["post-1078","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog","tag-bioinformatics","tag-genomics","tag-shotgun-sequencing"],"magazineBlocksPostFeaturedMedia":{"thumbnail":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2024\/05\/blogheader-1-150x150.jpg","medium":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2024\/05\/blogheader-1-300x225.jpg","medium_large":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2024\/05\/blogheader-1-768x576.jpg","large":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2024\/05\/blogheader-1-1024x768.jpg","1536x1536":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2024\/05\/blogheader-1-1536x1152.jpg","2048x2048":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2024\/05\/blogheader-1.jpg","colormag-highlighted-post":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2024\/05\/blogheader-1-392x272.jpg","colormag-featured-post-medium":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2024\/05\/blogheader-1-390x205.jpg","colormag-featured-post-small":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2024\/05\/blogheader-1-130x90.jpg","colormag-featured-image":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2024\/05\/blogheader-1-800x445.jpg","colormag-default-news":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2024\/05\/blogheader-1-150x150.jpg","colormag-featured-image-large":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2024\/05\/blogheader-1-1400x600.jpg"},"magazineBlocksPostAuthor":{"name":"onion","avatar":"https:\/\/secure.gravatar.com\/avatar\/a596197721c6296d27446d1377ed931e199d7d76ae4dedbebf95195b579de271?s=96&d=mm&r=g"},"magazineBlocksPostCommentsNumber":false,"magazineBlocksPostExcerpt":"\u5f9e\u6563\u5f48\u69cd\u7684FASTQ\u7522\u51fa\u300c\u4e00\u500b\u5708\u5708\u300d\u7684\u597d\u5de5\u5177","magazineBlocksPostCategories":["blog"],"magazineBlocksPostViewCount":75,"magazineBlocksPostReadTime":1,"magazine_blocks_featured_image_url":{"full":["https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2024\/05\/blogheader-1.jpg",1600,1200,false],"medium":["https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2024\/05\/blogheader-1-300x225.jpg",300,225,true],"thumbnail":["https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2024\/05\/blogheader-1-150x150.jpg",150,150,true]},"magazine_blocks_author":{"display_name":"onion","author_link":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/?author=1"},"magazine_blocks_comment":0,"magazine_blocks_author_image":"https:\/\/secure.gravatar.com\/avatar\/a596197721c6296d27446d1377ed931e199d7d76ae4dedbebf95195b579de271?s=96&d=mm&r=g","magazine_blocks_category":"blog<\/a>","_links":{"self":[{"href":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1078","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1078"}],"version-history":[{"count":10,"href":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1078\/revisions"}],"predecessor-version":[{"id":1232,"href":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1078\/revisions\/1232"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/index.php?rest_route=\/wp\/v2\/media\/1696"}],"wp:attachment":[{"href":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1078"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1078"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1078"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}\u95dc\u65bc\u786c\u9ad4<\/h3>\n\n\n\n
\u95dc\u65bc\u8edf\u9ad4\u5b89\u88dd\u7684\u65b9\u5f0f<\/h3>\n\n\n\n
>conda install -c bioconda unicycler<\/code><\/pre>\n\n\n\n>unicycler -1 short_reads_1.fastq.gz -2 short_reads_2.fastq.gz -l long_reads.fastq -o output_dir<\/code><\/pre>\n","protected":false},"excerpt":{"rendered":"