{"id":1099,"date":"2023-04-14T22:55:57","date_gmt":"2023-04-14T22:55:57","guid":{"rendered":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/?p=1099"},"modified":"2024-05-08T22:20:55","modified_gmt":"2024-05-08T22:20:55","slug":"nanopore-mk1c-%e8%bb%9f%e9%ab%94%e5%8d%87%e7%b4%9a%e4%ba%8c%e4%b8%89%e4%ba%8b","status":"publish","type":"post","link":"https:\/\/megagenome.nchu.edu.tw\/wordpress\/?p=1099","title":{"rendered":"Nanopore Mk1C \u8edf\u9ad4\u5347\u7d1a(2023\u5e74\u521d)"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">Certificate\u6191\u8b49\u904e\u671f\u6240\u4ee5\u4e0d\u80fd\u7528\u4e86\uff01\uff1f<\/h2>\n\n\n\n<!--more-->\n\n\n\n<figure class=\"wp-block-image alignright size-full is-resized is-style-default\"><a href=\"https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2023\/04\/MinKNOW2205.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2023\/04\/MinKNOW2205.jpg\" alt=\"Mk1C\" class=\"wp-image-1116\" style=\"width:512px;height:384px\" srcset=\"https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2023\/04\/MinKNOW2205.jpg 1024w, https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2023\/04\/MinKNOW2205-300x225.jpg 300w, https:\/\/megagenome.nchu.edu.tw\/wordpress\/wp-content\/uploads\/2023\/04\/MinKNOW2205-768x576.jpg 768w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/figure>\n\n\n\n<p>\u6709\u4e00\u5929\u4e2d\u5348\uff0c\u6211\u5011\u7814\u7a76\u751f\u628a\u525b\u505a\u597d\u7684library\u6ef4\u9032\u53bb\u4e00\u7247\u5148\u524dQC\u6c92\u904e\u7684flowcell\u8981\u505a\u5b9a\u5e8f\uff0c\u5979\u60f3\u8aaa\u6309\u4e0b\u53bb\u5c31\u53ef\u4ee5\u53bb\u5403\u98ef\u4e86\uff0c\u9019\u6642\u624d\u7a81\u7136\u767c\u73feMk1C\u4e0d\u80fd\u5b9a\u5e8f\u4e86\u3002\u64cd\u4f5c\u756b\u9762\u5361\u5728\u9078\u64c7kit\u7684\u5730\u65b9\uff0c\u300c\u7e7c\u7e8c\u4e0b\u4e00\u6b65\u300d\u7684\u6309\u9215\u4e0d\u7d66\u6309\u4e86\u3002<\/p>\n\n\n\n<p>\u72c0\u6cc1\u662f\uff0c\u9019\u53f0Mk1C\u4e0a\u7684MinKNOW\u7248\u672c\u9084\u572822.05.8 (bionic)\uff0c\u63a5\u4e0a\u7db2\u969b\u7db2\u8def\u4f3c\u4e4e\u90fd\u6709\u81ea\u52d5\u6aa2\u67e5\u8edf\u9ad4\u66f4\u65b0\uff0c\u4f46\u4e0d\u7ba1\u662fMinKNOW\u9084\u662f\u4f5c\u696d\u7cfb\u7d71\uff0c\u90fd\u6c92\u51fa\u73fe\u53ef\u4ee5\u66f4\u65b0\u8edf\u9ad4\u7684\u9078\u9805\u3002\u800c\u5b98\u7db2\u4e0aMinKNOW\u7684\u7248\u672c\u90fd\u5347\u7d1a\u523022.12.5\u4e86\uff0c\u9019\u7a76\u7adf\u662f\u600e\u9ebc\u56de\u4e8b\u5462\uff1f<\/p>\n\n\n\n<p>\u5176\u5be6\u793e\u7fa4<a rel=\"noreferrer noopener\" href=\"https:\/\/nanoporetech.com\" data-type=\"URL\" data-id=\"https:\/\/nanoporetech.com\" target=\"_blank\">\u5b98\u7db2<\/a>\u57282023\u5e74\u4e00\u6708\u4e0b\u65ec\u66fe\u6709\u8cbc\u904e\u4e00\u5247\u76f8\u95dc\u8aaa\u660e\uff0c\u4e0a\u9762\u7684\u89e3\u91cb\u5927\u6982\u662f\u8aaa\uff1a\u7531\u65bc\u6191\u8b49\u904e\u671f\uff0c\u4f7f\u7528\u8f03\u820a MinKNOW \u7248\u672c\uff08MinKNOW \u7248\u672c 22.05.8 \u6216\u66f4\u820a\uff09\u6703\u7121\u6cd5\u5b9a\u5e8f\u3002\u70ba\u4e86\u8b93MinKNOW \u53ef\u4ee5\u904b\u4f5c\uff0c\u4f60\u9700\u8981\u5347\u7d1a\u5230\u8f03\u65b0\u7684\u7248\u672c\u6216\u8a2d\u6cd5\u66f4\u65b0\u4f60\u88dd\u7f6e\u88e1\u7684\u6191\u8b49\u3002\u5426\u5247\u4f60\u7121\u6cd5\u6210\u529f\u5b8c\u6210flowcell\u7684QC\uff0c\u8981\u57f7\u884c\u5b9a\u5e8f\u6642\uff0c\u6703\u5728\u9078\u64c7kit\u8655\u5361\u4f4f\uff0c\u800c\u7121\u6cd5\u57f7\u884c\u5b9a\u5e8f\u3002<\/p>\n\n\n\n<p>\u597d\uff0c\u56e0\u70ba\u6211\u5011\u5728Mk1C\u7684\u5167\u5efa\u87a2\u5e55UI\u4e0a\u6c92\u8fa6\u6cd5\u9032\u884c\u5347\u7d1a\uff0c\u52a0\u4e0a\u5be6\u9a57\u5df2\u7d93\u7bad\u5728\u5f26\u4e0a\uff0c\u6240\u4ee5\u7576\u6642\u6211\u5011\u63a1\u53d6\u4e86\u300c\u6025\u6551\u300d\u7684\u505a\u6cd5\uff1a\u5148\u628a\u6191\u8b49\u88dd\u4e0a\u53bb\u3002<\/p>\n\n\n\n<p>\u5b89\u88dd\u65b0\u6191\u8b49\u7684\u6025\u6551\u4f5c\u6cd5\u5982\u4e0b\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u5230\u5b98\u7db2\u793e\u7fa4\u4e0b\u8f09\u65b0\u7684certificate file (new-certs.zip)\uff0cunzip\u4f60\u6703\u5f97\u5230\u5169\u500b\u6a94\u6848localhost.crt\u8207localhost.key\u3002<\/li>\n\n\n\n<li>\u7528SSH\u9023\u7dda\u5230Mk1C (\u9810\u8a2d\u7684\u5e33\u5bc6\u90fd\u662f&#8217;minit&#8217;)\uff0c\u627e\u5230\/opt\/ont\/minknow\/conf\/rpc-certs\uff0c\u628a\u88e1\u9762\u7684localhost.crt\u8207localhost.key\u66f4\u540d(\u7559\u5b58\u4ee5\u9632\u842c\u4e00)<\/li>\n\n\n\n<li>\u7528SFTP\u628a\u4e0b\u8f09\u5230\u7684\u65b0localhost.crt\u8207localhost.key\u4e1f\u5230rpc-certs\u5e95\u4e0b\u3002<\/li>\n\n\n\n<li>\u5728Mk1C\u4e0a\u91cd\u555fMinKNOW(flowcell\u90fd\u4e0d\u8981\u52d5\u5b83)\uff0c\u9019\u6a2322.05.8\u5c31\u53ef\u4ee5\u9806\u5229\u9032\u5165\u5b9a\u5e8f\u7a0b\u5e8f\u4e86\u3002<\/li>\n<\/ol>\n\n\n\n<p>\u9019\u6a23\u96d6\u7136\u53ef\u4ee5\u6025\u7528\uff0c\u9577\u9060\u4f86\u8aaa\u5c07\u4f86\u9084\u662f\u4e0d\u884c\u7684(\u65b0\u7684flowcell\u8ddfkit\u5c31\u8981\u5e2d\u6372\u800c\u4f86\u4e86)\uff0c\u6240\u4ee5\u5be6\u9a57\u9806\u5229\u5b8c\u6210\u5f8c\uff0c\u6211\u5011\u9084\u662f\u5f97\u66f4\u65b0\u5b83\u7684\u8edf\u9ad4\u624d\u884c\u3002<\/p>\n\n\n\n<p>\u8a3b\uff1a\u76ee\u524d\u5b98\u7db2\u793e\u7fa4\u90a3\u500b\u4e0b\u8f09new-certs.zip\u7684\u8f09\u9ede\u5df2\u7d93\u4e0b\u67b6\u4e86\u3002<\/p>\n\n\n\n<p><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u628aMk1C\u4e0a\u770b\u4f3c\u4e0d\u80fd\u5347\u7d1a\u7684MinKNOW\u5f9e22.05.8\u5347\u7d1a\u4e0a\u53bb<\/h2>\n\n\n\n<p>\u66f4\u65b0\u7684\u65b9\u6cd5\u5982\u4e0b(\u5b98\u7db2\u793e\u7fa4\u8cbc\u6587\u6559\u7684)\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Mk1C\u958b\u6a5f\uff0c\u63a5\u4e0a\u7db2\u8def<\/li>\n\n\n\n<li>\u627e\u53f0\u96fb\u8166\uff0c\u7528SSH\u9023\u7dda\u5230Mk1C (\u9810\u8a2d\u7684\u5e33\u5bc6\u90fd\u662f&#8217;minit&#8217;) \uff0c\u5728\u88e1\u9762\u57f7\u884c\u4ee5\u4e0b\u6307\u4ee4\uff1a<\/li>\n<\/ol>\n\n\n\n<pre class=\"wp-block-code\"><code>$ sudo apt update\n\n$ apt download minknow-core-minion-1c-offline\n\n$ dpkg-deb --extract minknow-core-minion-1c*.deb minknow <\/code><\/pre>\n\n\n\n<p>\u5176\u5be6\u9019\u88e1\u5b98\u7db2\u8cbc\u6587\u5beb\u6210dpkg-deb &#8211;extract minknow-core-minion-nc_*.deb minknow\uff0c\u90a3\u6a23\u932f\u5f97\u5f88\u660e\u986f\uff0c\u7167\u8457\u505a\u7576\u7136\u4e0d\u884c\uff0c\u4f60\u4e0d\u80fd\u4e0d\u7528\u8166\u4ec0\u9ebc\u6307\u4ee4\u90fd\u4e0a\u7db2\u526a\u8cbc\u96a8\u4fbf\u7167\u8457\u786c\u5e79\u5440\uff01<\/p>\n\n\n\n<p>\u63a5\u4e0b\u4f86\uff1a<\/p>\n\n\n\n<pre class=\"wp-block-code\"><code>$ sudo mv -fv minknow\/opt\/ont\/minknow\/conf\/rpc-certs\/localhost.* \/opt\/ont\/minknow\/conf\/rpc-certs\/\n\n$ rm -fr minknow\n\n$ sudo systemctl restart minknow.service<\/code><\/pre>\n\n\n\n<p>\u9019\u6a23\u6703\u91cd\u65b0\u555f\u52d5MinKNOW\uff0c\u5982\u679cMk1C\u4e0a\u7684MinKNOW\u5361\u5728\u300c\u8981\u4e0d\u8981\u91cd\u555f\u300d\uff0c\u5c31\u6309\u4e0b\u53bb\u91cd\u555fMinKNOW\u5427\uff01<\/p>\n\n\n\n<p>\u4f60\u53ef\u4ee5\u628aMk1C\u91cd\u958b\u6a5f\uff0c\u770b\u770b\u662f\u5426\u4e00\u5207\u6b63\u5e38\u3002<\/p>\n\n\n\n<p><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u4ee5\u4e0b\u662f\u53ef\u80fd\u6703\u5361\u4f4f\u7684\u5730\u65b9\uff0c\u4f46\u662f\u5b98\u7db2\u6c92\u6709\u6559\u7684\uff1a<\/h2>\n\n\n\n<p>\u5176\u5be6\u5728\u4e00\u958b\u59cb\u7684sudo apt update\u5c31\u53ef\u80fd\u6703\u5361\u4f4f\u4e86\uff01\u57f7\u884csudo apt update\u53ef\u80fd\u6703\u51fa\u73fe\u542b\u6709\u9019\u6a23\u5167\u5bb9\u7684\u932f\u8aa4\u8a0a\u606f\uff1a<\/p>\n\n\n\n<pre class=\"wp-block-code\"><code>Err:3 https:\/\/cdn.oxfordnanoportal.com\/apt bionic-stable-mk1c InRelease\n  The following signatures couldn't be verified because the public key is not available: NO_PUBKEY <\/code><\/pre>\n\n\n\n<pre class=\"wp-block-code\"><code>W: An error occurred during the signature verification. The repository is not updated and the previous index files will be used. GPG error: https:\/\/cdn.oxfordnanoportal.com\/apt bionic-stable-mk1c InRelease: The following signatures couldn't be verified because the public key is not available: NO_PUBKEY 94565B8938B1CC97<\/code><\/pre>\n\n\n\n<p>\u6240\u8b02APT\uff08Advanced Packaging Tool\uff09\u662f Ubuntu (Mk1C\u7684\u4f5c\u696d\u7cfb\u7d71) \u4e2d\u7ba1\u7406\u8edf\u9ad4\u5957\u4ef6\u7684\u5de5\u5177\u3002\u7576\u4f60\u57f7\u884c sudo apt-get \u547d\u4ee4\u6642\uff0c\u5b83\u6703\u8ca0\u8cac\u6aa2\u67e5\u7cfb\u7d71\u4e2d\u914d\u7f6e\u7684\u8edf\u9ad4\u5957\u4ef6\u5eab\uff0c\u7136\u5f8c\u5e6b\u4f60\u4e0b\u8f09\u3001\u5b89\u88dd\u6216\u5347\u7d1a\u5957\u4ef6\u53ca\u5176\u76f8\u4f9d\u5957\u4ef6\u3002\u7576\u4f60\u5728\u4f7f\u7528 sudo apt-get \u547d\u4ee4\u6642\uff0c\u5982\u679c\u51fa\u73fe &#8220;NO_PUBKEY&#8221; \uff08\u627e\u4e0d\u5230public key\uff09\u7684\u932f\u8aa4\u8a0a\u606f\uff0c\u9019\u901a\u5e38\u662f\u7531\u65bc APT \u7121\u6cd5\u9a57\u8b49\u5957\u4ef6\u7684\u771f\u5be6\u6027\uff0c\u9019\u7a2e\u60c5\u6cc1\u767c\u751f\u7684\u539f\u56e0\uff0c\u901a\u5e38\u662f\u56e0\u70ba\u4f60\u8981\u7528\u7684\u5957\u4ef6\u5eab\u7684 GPG \u91d1\u9470\u6c92\u6709\u88ab\u6dfb\u52a0\u5230\u4f60\u7684\u7cfb\u7d71\uff08\u6bd4\u8f03\u7cdf\u7cd5\u4e00\u9ede\u6216\u8a31\u662f\u5957\u4ef6\u5eab\u88ab\u99ed\u5ba2\u7d66\u60e1\u641e\u4e86\uff09\u3002\u8981\u89e3\u6c7aNO_PUBKEY\u554f\u984c\uff0c\u4f60\u9700\u8981\u5c07\u5957\u4ef6\u5eab\u7684 GPG \u91d1\u9470\u5c0e\u5165\u4f60\u7684\u7cfb\u7d71\u4e2d\u3002\u5176\u5be6\u4f60\u53ef\u4ee5\u5728\u5957\u4ef6\u5eab\u7684\u7db2\u7ad9\u6216\u91d1\u9470\u4f3a\u670d\u5668\u4e0a\u627e\u5230\u91d1\u9470\uff0c\u7136\u5f8c\u4f7f\u7528 apt-key \u547d\u4ee4\u5c07\u5176\u5c0e\u5165\u4f60\u7684\u7cfb\u7d71\u3002\u4ee5\u4e0a\u9762\u7684\u932f\u8aa4\u8a0a\u606f\u4f86\u8aaa\uff0c\u5b83\u662f\u53eb94565B8938B1CC97\u7684PUBKEY\uff0c\u6240\u4ee5\u6211\u5011\u7684\u89e3\u6c7a\u65b9\u6cd5\u5982\u4e0b\uff1a<\/p>\n\n\n\n<pre class=\"wp-block-code\"><code>$ sudo apt-key adv --keyserver keyserver.ubuntu.com --recv-keys 94565B8938B1CC97<\/code><\/pre>\n\n\n\n<p>\u505a\u5b8c\u9019\u500b\u52d5\u4f5c\u4e4b\u5f8c\uff0c\u4f60\u5c31\u53ef\u4ee5\u9806\u5229\u9032\u884c sudo apt 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